Research Lab Tech 1 (Chekuri)

POSITION SUMMARY:

The Grousbeck Gene Therapy Center at Mass Eye and Ear has an open position for a Research Assistant to assist in the application of novel gene therapy modalities. The Center provides a dynamic multi-disciplinary environment that pursues the common goal of developing therapeutic gene transfer in future medicine.

We are seeking a Research Assistant to join the laboratory of Dr. Anil Chekuri to perform in vivo experiments both in small animal models. The main responsibilities of this position will include animal experimentation and rodent colony management. Familiarity with tissue processing is desirable. Demonstrated excellence in time management and organizational skills are essential. A bachelor's degree in biology or related discipline is required.

We offer a flexible and collaborative environment, as well as the opportunity to develop scientifically and explore the promising field of translational AAV gene therapy.

ESSENTIAL FUNCTIONS:

* Animal work (rodents):

- Colony management and animal handling (breeding, tagging, and genotyping)

- Sub-retinal injections

- Injections (SQ, IM, RO, IV, IVT, SR, IP)

- In vivo imaging and Electroretinography

- Pre-op and post-op care

- Euthanasia/necropsy

- Tissue collection

* Ensure cleanliness of the lab and equipment including instrument maintenance.

* Adherence to pertinent departmental policies, practices and procedures (SOPs, IACUC, Hazardous materials, safety procedures and biosafety protocols).

* Keep accurate records of experiments; analyze data, and present results to PI.

* Tissue processing (tissue fixation/freezing, embedding, cryo-sectioning and DNA/RNA extraction) is an asset.

* Molecular cloning of plasmids using PCR, restriction digestion and other methods

* Cell and tissue culture.

* Biochemical assays including SDS-PAGE and ELISA.

EDUCATION AND EXPERIENCE:

• BS/BA in biology preferred or related discipline
• Experience in or willing to learn vivo rodent manipulation (including colony management, injections and necropsies).
• Experience in or willing to learn Optical coherence tomography, fundus imaging, and in vivo bioluminescence imaging.
• Must be able to work as part of a team.
• Excellent time management and organization.

WORKING CONDITIONS:

Work will primarily take place in a research laboratory setting. Job may require exposure to bio-hazardous or toxic substances.

PHYSICAL REQUIREMENTS:

Position requires alertness and manual dexterity.

Massachusetts Eye and Ear is proud to be an equal-opportunity employer and is committed to providing a workplace free from harassment or discrimination. All employment decisions are made without regard to race, color, age, gender, gender identification, sexual orientation, religion, marital status, sex, pregnancy or conditions related to pregnancy, national origin/ancestry, citizenship, disability, military status, genetic information, or any other basis prohibited by law. These protections extend to all management practices and decisions, including recruitment and hiring practices, appraisal systems, promotions, training, and career development programs.